Composite

Part:BBa_M33355:Design

Designed by: Alejandro Virrueta   Group: Stanford BIOE44 - S010   (2010-06-06)


pTod + Purple Color Generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1153
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5726
    Illegal BamHI site found at 4413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3938
    Illegal AgeI site found at 4134
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 6676
    Illegal SapI.rc site found at 6751


Design Notes

None


Source

The sequence of the Ptod promoter was given in the article referenced below. This identicle sequence was found upstream of the todX gene sequence natural existent in Pseudomonas putida given in http://www.ncbi.nlm.nih.gov/nuccore/AB042508.1 (todX coordinates: 14325..15686).

References